Journal: Current biology : CB

Article Title: Developmentally arrested precursors of pontine neurons establish an embryonic blueprint of the Drosophila central complex

doi: 10.1016/j.cub.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ Source Identifier Antibodies Rat anti-DN-cadherin Developmental Studies Hybridoma Bank DN-EX #8, RRID: AB_528121 Mouse anti-Bruchpilot Developmental Studies Hybridoma Bank nc82, RRID: AB_2314866 Mouse anti-Neurotactin Developmental Studies Hybridoma Bank BP106, RRID: AB_528404 Mouse anti-Neuroglian Developmental Studies Hybridoma Bank BP104, RRID: AB_528402 Mouse 8D12 anti-Repo Developmental Studies Hybridoma Bank RRID: AB_528448 Rabbit anti-β-galactosidase Cappel/ICN (now MP Biomedicals) Cat #: 55976 Rabbit anti-HA Cell Signaling Technologies Cat#: mAb #3724 Chicken anti-GFP Invitrogen, Thermo Fisher Scientific Cat#: A10262 Rat anti-FLAG Novus Biologicals Cat#: NBP1–06712SS Goat anti-Mouse Alexa Flour-568 Life Technologies, Thermo Fisher Scientific Cat#: A-11031 Donkey anti-Mouse Cy5 Jackson Immunoresearch, Molecular Probes Code: 712-175-153 Donkey anti-Rat Cy3 Jackson Immunoresearch, Molecular Probes Code: 712-165-153 Goat anti-Rat Cy5 Jackson Immunoresearch, Molecular Probes Code: 112-175-143 Goat anti-Rabbit Cy3 Jackson Immunoresearch, Molecular Probes Code: 711-165-152 Goat anti-Rabbit Alexa Flour-488 Invitrogen, Thermo Fisher Scientific Cat#: A-11008 Goat anti-Chicken Alexa Flour-488 Thermo Fisher Scientific Cat#: A-11039 Experimental Models: Organisms/ Strains w[1118];P{y[+t7.7]w[+mC]=GMR45F08-GAL4} attP2 Bloomington Stock Centre (USA) BL#49565 w[1118];P{y[+t7.7]w[+mC]=10XUAS-mCD8::GFP} attP2 Bloomington Stock Centre (USA) BL#32184 w[*]:P{y[+t7.7]w[+mC]=10XUAS-IVS-mCD8::GFP} attP40 Bloomington Stock Centre (USA) BL#32186 w[1118];P{y[+t7.7]w[+mC]=GMR57C10-FLP2.PEST}su(Hw)attP8;PBac{[+mDint2]w[+mC]=10xUAS(FRT.stop)myr::smGdP-HA}VK00005P{y[+t7.7]w[+mC]=10xUAS(FRT.stop)myr::smGdP-V5-THS-10xUAS(FRT.stop)myr::smGdp-FLAG}su(Hw)attP1 Gift from Mark Frye Lab [ 48 ] UAS-his UAS-rpr-UAS-lacZ [ 48 ] w[*];sna[Sco/CyO; P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7] Bloomington Stock Centre (USA) BL#7018 Drl-tau::lacZ Prof. H. Hing (The College at Brockport, State University of New York) [ 49 ] Chemicals Paraformaldehyde 16% Solution, EM Grade Electron Microscopy Sciences Cat#: 15710 Triton X-100 MP Biomedicals, LLC Cat#: 194854 Agar Sigma-Aldrich A1296 Software Image-J/Fiji Fiji N/A Adobe Illustrator Adobe System N/A Adobe Photoshop Adobe System N/A CATMAID CATMAID.org [ 52 ] Open in a separate window KEY RESOURCES TABLE Many early larval neurons lack a branched neurite tree and synapses (SU neurons) Axons of SU neurons form bundles associated with the fiber tracts of most lineages SU neurons of lineages DM1-DM4 pioneer the neural architecture of the central complex SU neurons differentiate into the pontine neurons of the adult central complex

Techniques: Electron Microscopy, Software